Download Experiments in Textile and Fibre Chemistry by Christopher Earland PDF

By Christopher Earland

ISBN-10: 0408700890

ISBN-13: 9780408700894

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The paper must be handled at the edges only, since finger marks show up as confusing spots after spraying with the very sensitive ninhydrin locating agent. The drop of solution applied to the paper contains only about 5 |ag (1 \xg = 1 microgramme = 10" g ) of any individual amino acid. Lay the square of paper on another sheet of clean paper so that the origin line hangs over the edge of the bench. Apply the solutions to the paper using a piece of capillary (melting point) tube, washing the tube thoroughly each time to avoid cross-contamination.

46, 2 7 ( 1 9 5 0 ) 17 Determination of the tyrosine content of a protein APPARATUS Spekker photo-electric absorptiometer. No. 3 porosity sinteredglass funnels. MATERIALS Mercuric sulphate, mercuric chloride, anhydrous sodium sulphate, sodium nitrite, tyrosine, samples of wool and silk. THEORY Millon's reagent (a solution of mercury in nitric acid) for phenols was employed by Hoffmann in 1853 for testing for tyrosine. The test depends on the presence of a little nitrous acid in the solution and it is generally accepted that the red colour is due to chelation between the nitrosophenol and the mercury.

Add D N S chloride reagent in acetone (2 ml) and allow to stand for 3 h at room temperature. Add acetone (10 ml) and filter off the precipitated sodium bicarbonate. Evaporate to dryness in an evacuated desiccator over silica gel and dissolve the residue in 50 per cent aq. acetone (2 ml) for chromatographic examination. NOTES 1. DNS-amino acids are similar to D N P derivatives in that they are very susceptible to photodegradation. Wherever possible, if the material is allowed to stand, light should be excluded.

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Experiments in Textile and Fibre Chemistry by Christopher Earland

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